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The developmental timing of spinal touch processing alterations and its relation to ASD-associated behaviors in mouse models 
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a , Experimental setup for perinatal mechanoreceptor optical activation assay. P0 or E18.5 mice are placed on a clear acrylic stage, and LED illumination is directed to the paw or back hairy skin. Parts created with BioRender.com . b , Average displacement responses to 5 optical stimuli (20-30 second ISIs) delivered to mechanoreceptor subtypes in forepaw, back hairy skin, and hindpaw compared to control littermates that lack opsin expression (controls include opsin-positive, Cre-negative and opsin-negative, Cre-positive animals). c , Average displacement responses to 5 optical stimuli activating Ret + Aβ LTMRs to the forepaws of P0 Ret CreER ; Advillin FlpO ; <t>Rosa26</t> LSL-FSF-ReaChR-mCitrine ; Gabrb3 +/+ and Gabrb3 +/- animals. P =0.0169, unpaired one-tailed Welch’s t test. d , Example displacement traces from a E18.5 Gabrb3 +/+ mouse and Gabrb3 +/- mouse to optical activation of Ret + Aβ LTMRs in the forepaw. e , Average displacement responses to 5 optical stimuli activating Ret + Aβ LTMRs in the forepaws of E18.5 control and mutant animals. P =0.0029, unpaired one-tailed Welch’s t test. For b , c and e , data represent means±s.e.m.
The developmental timing of spinal touch processing alterations and its relation to ASD-associated behaviors in mouse models 
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Related to . a, Anatomical labeling of the corticospinal tract in thoracic spinal cords of P0, P4, and adult animals labeled by in Emx1 Cre ; <t>Rosa26</t> <t>LSL-synaptophysin-tdTomato</t> . Scale bar denotes 200 µm. Parts created with BioRender.com . b, Example miniature excitatory post-synaptic currents (mEPSCs) from P4 and P19-21 spinal cord interneurons of lamina III/IV (top), and quantification of frequencies (bottom, left) and amplitudes (bottom, right). N=2 animals for P4 and N=3 for P19-21. For frequencies, P =0.0929, Mann-Whitney U test. For amplitudes, P =0.6822, unpaired two-tailed t-test. c, Spinal cord immunohistochemistry showing expression of VGAT-labeled synaptic terminals and GLYRα1 in P3-P4 and adult mice. Scale bar denotes 10 µm. d, Quantification of VGAT and GLYRα1 co-labeled puncta, as a fraction of total VGAT+ puncta in a field of view, at P3-P4 or adulthood. P <0.0001, unpaired two-tailed t-test. For b and d, data represent means±s.e.m.
The developmental timing of spinal touch processing alterations and its relation to ASD-associated behaviors in mouse models bioRxiv, 2023 May 09
"a , Experimental setup for perinatal mechanoreceptor optical activation assay. P0 or E18.5 mice are placed on a clear acrylic stage, and LED illumination is directed to the paw or back hairy skin. Parts created with BioRender.com . b , Average displacement responses to 5 optical stimuli (20-30 second ISIs) delivered to mechanoreceptor subtypes in forepaw, back hairy skin, and hindpaw compared to control littermates that lack opsin expression (controls include opsin-positive, Cre-negative and opsin-negative, Cre-positive animals). c , Average displacement responses to 5 optical stimuli activating Ret + Aβ LTMRs to the forepaws of P0 Ret CreER ; Advillin FlpO ; <t>Rosa26</t> LSL-FSF-ReaChR-mCitrine ; Gabrb3 +/+ and Gabrb3 +/- animals. P =0.0169, unpaired one-tailed Welch’s t test. d , Example displacement traces from a E18.5 Gabrb3 +/+ mouse and Gabrb3 +/- mouse to optical activation of Ret + Aβ LTMRs in the forepaw. e , Average displacement responses to 5 optical stimuli activating Ret + Aβ LTMRs in the forepaws of E18.5 control and mutant animals. P =0.0029, unpaired one-tailed Welch’s t test. For b , c and e , data represent means±s.e.m. "
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